Revalesio scientists and their collaborators at Rush University reported that RNS60 elicited anti-inflammatory effects in microglia and astroglia activated by inflammatory stimuli. RNS60 inhibited the activation of NF-κB, an important target for controlling inflammation, and thereby the production of nitric oxide (NO). RNS60 achieved this anti-inflammatory effect through up-regulation of an endogenous inhibitor of NF-κB called IκBα via the PI 3-kinase-Akt pathway. These data provided the first explanation for the anti-inflammatory properties of RNS60 and laid the foundation for its development as an anti-inflammatory and neuroprotective treatment.

Link to the paper: Suppression of Nuclear Factor-κB Activation and Inflammation in Microglia by Physically Modified Saline* – Journal of Biological Chemistry (jbc.org)